| MLO | Phase separation |
|---|---|
| Class | LLPS |
| Synonyms | Phase transition, Liquid-liquid phase separation |
| Classification | nervous system disease |
|---|---|
| Disease | Amyotrophic lateral sclerosis |
| More Disease Information | |
| MLO change | Size: - | Number: - | Assembly: - | ||
|---|---|---|---|---|---|
| Dynamic: - | Others: - | ||||
| Description: - | |||||
| Publication note | Although best known for its predominantly disordered C-terminal domain which mediates ALS inclusions,TDP-43 has a globular N-terminal domain (NTD). TDP-43 NTD assembles into head-to-tail linear chains and that phosphomimetic substitution at S48 disrupts TDP-43 polymeric assembly,discourages liquid-liquid phase separation (LLPS) in vitro,fluidizes liquid-liquid phase separated nuclear TDP-43 reporter constructs in cells,and disrupts RNA splicing activity. | ||||
| Organism | Homo sapiens | Cell line or tissue | HEK293T | ||
| Experiment evidence | Indirect experiment | Reference | 29438978 | ||
| In vivo PS evidence | FRAP: No | 1,6-hexa treatment: No | Spherical shape: Yes | ||
| Fusion&Fission: No | PS role: Self | ||||
| Mutation | - | Expression change | - |
|---|---|---|---|
| PTM | Position Residue | Modification | Modifying enzyme |
| - | - | - | |
| Description | Mutation: - Expression change: - PTM: A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation, and RNA splicing |
||
| Drugs from DrugBank | - | |
|---|---|---|
| Molecules from BindingDB(PubChem CID) | 45223816；121380360；56739767；863；4212；6112 | |
| Molecules from PDBBind | - | |
| Interference Note | - |
| Pscore overall score | 5.95 | PSPer | 0.38 |
|---|---|---|---|
| catGRANULE overall score | 2.04 | R+Y | 0.0018461538000000002 |
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Chao Hou, Haotai Xie, Yang Fu, Yao Ma, Tingting Li. MloDisDB: A manually curated DataBase of the relations between MembraneLess Organelles and DISeases