| MLO | Nucleolus |
|---|---|
| Class | MLO-unchanged |
| Synonyms | Nucleoli |
| Classification | cancer |
|---|---|
| Disease | Breast cancer |
| More Disease Information | |
| MLO change | Size: - | Number: - | Assembly: - | ||
|---|---|---|---|---|---|
| Dynamic: - | Others: - | ||||
| Description: - | |||||
| Publication note | Activated PARP-1 ADP-ribosylates DDX21,an RNA helicase that localizes to nucleoli and promotes rDNA transcription when ADP-ribosylated. Treatment with PARPi or mutation of the ADP-ribosylation sites reduces DDX21 nucleolar localization,rDNA transcription,ribosome biogenesis,protein translation,and cell growth.The salient features of this pathway are evident in xenografts in mice and human breast cancer patient samples. Elevated levels of PARP-1 and nucleolar DDX21 are associated with cancer-related outcomes. Our studies provide a mechanistic rationale for efficacy of PARPi in cancer cells lacking defects in DNA repair whose growth is inhibited by PARPi. | ||||
| Organism | Homo sapiens,Mus musculus | Cell line or tissue | MCF-7,MDA-MB-231,T47D,HCC1143,MDA-MB-436,293T,HMEC | ||
| Experiment evidence | Indirect experiment | Reference | 31351877 | ||
| In vivo PS evidence | FRAP: - | 1,6-hexa treatment: - | Spherical shape: - | ||
| Fusion&Fission: - | PS role: - | ||||
| Mutation | ADPRylation site mutant | Expression change | - |
|---|---|---|---|
| PTM | Position Residue | Modification | Modifying enzyme |
| - | - | - | |
| Description | Mutation: Treatment with PARPi or mutation of the ADP-ribosylation sites reduces DDX21 nucleolar localization, rDNA transcription, ribosome biogenesis, protein translation, and cell growth. Expression change: - PTM: - |
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| Drugs from DrugBank | - | |
|---|---|---|
| Molecules from BindingDB(PubChem CID) | 145950109 6077469 23133376 30293181 55537391 6226266 5006184 5128834 5190750 6230328 62666779 37637241 0999499 7397575 55526650 4402172 53383 6225808 5218503 804416 0928352 607850 5190718 37636259 64622885 857216 5218485 4542060 4549902 821065 45966106 43 | |
| Molecules from PDBBind | 1uk0 1uk1 1wok 2rcw 2rd6 3gjw 3gn7 3l3l 3l3m 4hhy 4hhz 4l6s 4pjt 4r5w 4r6e 4und 4uxb 4zzz 5a00 5ha9 5xsr 5xst 5xsu 6bhv 6nrf 6nrg 6nrh 6nri 6nrj | |
| Interference Note | - |
| Pscore overall score | 1.3 | PSPer | 0.21 |
|---|---|---|---|
| catGRANULE overall score | 1.37 | R+Y | 0.0 |
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Chao Hou, Haotai Xie, Yang Fu, Yao Ma, Tingting Li. MloDisDB: A manually curated DataBase of the relations between MembraneLess Organelles and DISeases