| MLO | Phase separation |
|---|---|
| Class | LLPS |
| Synonyms | - |
| Factor type | protein |
|---|---|
| Factor name | NCAP_SARS2 |
| NCBI Gene ID | 43740575 |
| UniProt ID | P0DTC9 |
| Classification | other diseases |
|---|---|
| Disease | COVID-19 |
| More Disease Information | |
| Disease | COVID-19 |
|---|---|
| Disease Ontology | DOID:0080600 |
| MeSH | D000086382 |
| ICD-10-CM | U07.1 |
| OMIM |
| MLO change | Size: - | Number: - | Assembly: - | ||
|---|---|---|---|---|---|
| Dynamic: - | Others: - | ||||
| Description: We found that RNAs triggered the robust LLPS of N protein both in vitro and in vivo. Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), which is shared by all the sub-genome mRNAs8, resulted in the formation of N protein condensates. | |||||
| Publication note | We found that RNAs triggered the robust LLPS of N protein both in vitro and in vivo. The deletion of any domains, including CTD, NTD and IDRs, disrupted the RNA-binding and LLPS ability of N protein. R203K/G204R gained greater ability to undergo LLPS in v | ||||
| Organism | Homo sapiens | Cell line or tissue | A549, H1299 | ||
| Experiment evidence | Direct experiment | Reference | 33837182 | ||
| In vivo PS evidence | FRAP: Yes | 1,6-hexa treatment: No | Spherical shape: No | ||
| Fusion&Fission: Yes | PS role: Client | ||||
| Mutation | R203K, G204R | Expression change | - |
|---|---|---|---|
| PTM | Position Residue | Modification | Modifying enzyme |
| - | - | - | |
| Description | Mutation: The polymorphism of R203K/G204R, which exhibited a higher propensity to undergo LLPS in the presence of RNAs, showed a greater effect on the inhibition of IFN expression. Expression change: - PTM: - |
||
| Drugs from DrugBank | - | |
|---|---|---|
| Molecules from BindingDB(PubChem CID) | - | |
| Molecules from PDBBind | - | |
| Interference Note | We then examined the LLPS of N protein with the application of increasing concentrations of GCG, the results showed that 12.5 μM was sufficient to block the N protein LLPS. We further titrated the concentrations of GCG below 10 μM and found that 6–8 μM we |
| Pscore overall score | - | PSPer | |
|---|---|---|---|
| catGRANULE overall score | - | R+Y | |
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Chao Hou, Haotai Xie, Yang Fu, Yao Ma, Tingting Li. MloDisDB: A manually curated DataBase of the relations between MembraneLess Organelles and DISeases